Immobilization of 3 yeasts in a commercial, polyvinyl alcohol (PVA) gel bead system, and its effects on cell cell viability and heterologous protein production are described. The yeasts Saccharomyces cerevisiae, Kluyveromyces lactis and Zygosaccharomyces bailii were immobilized using the Lentikats® system. All 3 species remained viable after immobilization, reaching a max. immobilized biomass of 100 mg dry wt. cells/cm3. The beads were mechanically stable, and could be used in stirred-tank bioreactors. Continuous production of glycoamylase (glucan 1,4-α-glucosidase) by K. lactis and interleukin 1-beta by Z. bailii were demonstrated.
PVA-GEL (LENTIKATS) AS A NEW AND EFFECTIVE MATRIX FOR YEAST CELL IMMOBILIZATION AIMED AT HETEROLOGOUS PROTEIN PRODUCTION.
PARASCANDOLA, Palma;
2006-01-01
Abstract
Immobilization of 3 yeasts in a commercial, polyvinyl alcohol (PVA) gel bead system, and its effects on cell cell viability and heterologous protein production are described. The yeasts Saccharomyces cerevisiae, Kluyveromyces lactis and Zygosaccharomyces bailii were immobilized using the Lentikats® system. All 3 species remained viable after immobilization, reaching a max. immobilized biomass of 100 mg dry wt. cells/cm3. The beads were mechanically stable, and could be used in stirred-tank bioreactors. Continuous production of glycoamylase (glucan 1,4-α-glucosidase) by K. lactis and interleukin 1-beta by Z. bailii were demonstrated.File in questo prodotto:
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