Objectives: The objective of this work was to assess the reliability of a newly developed FTIR (Fourier Transform Infrared spectroscopy) technique for 13C-urea breath test in the non-invasive diagnosis of Helicobacter pylori infection, in comparison to those currently used, isotope ratio mass spectrometry (IRMS) and non-dispersive isotope-selective infrared spectrometry (NDIRS). This new methodology is based on the use of a very less expensive and sophisticated FTIR than IRMS and NDIRS. Materials and Methods: Sixty patients (male and female, aged between 15e70 years) with dyspeptic symptoms, like the epigastric pain, were tested for H. pylori infection using 13C-urea breath test. Triplicate breath samples were collected before and 30 min after drinking the test solution (75 mg 13C-urea dissolved in 200 ml 0.1 M citric acid). Analysis of d13C were conducted in parallel by means of IRMS, NDIRS and FTIR in order to compare the results. Results: Delta-over-baseline values were over 5& in 18 patients, ranging from 8.81 to 60.88& for IRMS measurements, from 7.2 to 61.2& for NDIRS and from 7.7 to 61.2& for FTIR measurements. The remaining 42 subjects had values well below the 5& cut-off. The results of the baseline-corrected 13CO2 exhalation values between FTIR and IRMS/NDIRS were in good agreement. Conclusions: The newly developed FTIR methodology has been demonstrated to be a reliable and accurate analytical tool, low cost and easy-to-operate, which permits a highly specific measurement of 13C enrichment in breath samples. As IRMS and NDIRS it allows to adequately discriminate between infected and non-infected subjects.
New FT-IR methodology for the evaluation of 13C/12C isotope ratio in Helicobacter pylori infection diagnosis
MOTTA, ORIANA;DE CARO, Francesco;PROTO, Antonio
2009-01-01
Abstract
Objectives: The objective of this work was to assess the reliability of a newly developed FTIR (Fourier Transform Infrared spectroscopy) technique for 13C-urea breath test in the non-invasive diagnosis of Helicobacter pylori infection, in comparison to those currently used, isotope ratio mass spectrometry (IRMS) and non-dispersive isotope-selective infrared spectrometry (NDIRS). This new methodology is based on the use of a very less expensive and sophisticated FTIR than IRMS and NDIRS. Materials and Methods: Sixty patients (male and female, aged between 15e70 years) with dyspeptic symptoms, like the epigastric pain, were tested for H. pylori infection using 13C-urea breath test. Triplicate breath samples were collected before and 30 min after drinking the test solution (75 mg 13C-urea dissolved in 200 ml 0.1 M citric acid). Analysis of d13C were conducted in parallel by means of IRMS, NDIRS and FTIR in order to compare the results. Results: Delta-over-baseline values were over 5& in 18 patients, ranging from 8.81 to 60.88& for IRMS measurements, from 7.2 to 61.2& for NDIRS and from 7.7 to 61.2& for FTIR measurements. The remaining 42 subjects had values well below the 5& cut-off. The results of the baseline-corrected 13CO2 exhalation values between FTIR and IRMS/NDIRS were in good agreement. Conclusions: The newly developed FTIR methodology has been demonstrated to be a reliable and accurate analytical tool, low cost and easy-to-operate, which permits a highly specific measurement of 13C enrichment in breath samples. As IRMS and NDIRS it allows to adequately discriminate between infected and non-infected subjects.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
