An immobilization method based on the spontaneous adhesion of invertase-active cells of yeast (Saccharomyces cerevisiae) to tuff granules was described by Parascandola, Scardi, and Tartaglione. Compared with gel entrapment, immobilization by adhesion is much more simple and free from diffusional limitations. However, adhesion is a rather complicated process involving surface interactions between microbial cells and the so-called substratum, that is, the solid support to which they attach. Because there are still many unanswered questions about the mechanism of adhesion, the selection of suitable substrata for a given microbial species can be made only empirically. Thus, to find substrata better than tuff or insolubilized gelatin, polymeric hydrogels that were obtained by radiation-induced polymerization below 0 °C and that were employed successfully for immobilizing enzymes, cells and antibodies were considered. A dozen of such polymer matrices with different hydrophilicities were synthesized and assayed as possible substrata for S. cereviisae cells used in continuous ethanol production.
Immobilization of Saccharomyces cerevisiae cells by adhesion to polymeric matrices obtained by radiation-induced polymerization
PARASCANDOLA, Palma;
1990-01-01
Abstract
An immobilization method based on the spontaneous adhesion of invertase-active cells of yeast (Saccharomyces cerevisiae) to tuff granules was described by Parascandola, Scardi, and Tartaglione. Compared with gel entrapment, immobilization by adhesion is much more simple and free from diffusional limitations. However, adhesion is a rather complicated process involving surface interactions between microbial cells and the so-called substratum, that is, the solid support to which they attach. Because there are still many unanswered questions about the mechanism of adhesion, the selection of suitable substrata for a given microbial species can be made only empirically. Thus, to find substrata better than tuff or insolubilized gelatin, polymeric hydrogels that were obtained by radiation-induced polymerization below 0 °C and that were employed successfully for immobilizing enzymes, cells and antibodies were considered. A dozen of such polymer matrices with different hydrophilicities were synthesized and assayed as possible substrata for S. cereviisae cells used in continuous ethanol production.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
