We previously identified apoptosis-induced regulator (air) as a pro-apoptotic transcript whose expression was repressed by NF-κB/Rel activity in the human leukemia cell line Jurkat (Turco, Lamberti, Bisogni, Romano, Petrella, Ammirante, Rosati, d'Avenia, Arra, Spugnini, Venuta, 2007, Leukemia 21:2557-2559). In this paper, we report that air sequence is detectable by Southern blot in human healthy donors (HHD) leukocytes and in two pancreatic adenocarcinoma cell lines (AsPC-1 and PANC-1), providing the first definitive evidence of air gene presence in human genome. In addition, we demonstrate that air expression is induced in the tumor cell lines by a naturally occurring ROS-inducing compound, phenethyl isothiocyanate (PEITC), a potential dietary cancer chemopreventive agent. Since PEITC inhibits NF-κB activation, air induction by this agent is consistent with the suppressive effect of NF-κB on air expression. This finding contributes a new clue to the role of NF-κB in regulating oxidative stress-induced pro-apoptotic genes and identifies a novel potential tool for enhancing pancreas cancer response to treatment.
The expresssion of the pro-apoptotic gene air is inducibile in human pancreatic adenocarcinoma cells
ROSATI, ALESSANDRA;BELISARIO, MARIA ANTONIETTA;TORINO, Gaetano;TURCO, Maria Caterina;PASCALE, Maria
2011-01-01
Abstract
We previously identified apoptosis-induced regulator (air) as a pro-apoptotic transcript whose expression was repressed by NF-κB/Rel activity in the human leukemia cell line Jurkat (Turco, Lamberti, Bisogni, Romano, Petrella, Ammirante, Rosati, d'Avenia, Arra, Spugnini, Venuta, 2007, Leukemia 21:2557-2559). In this paper, we report that air sequence is detectable by Southern blot in human healthy donors (HHD) leukocytes and in two pancreatic adenocarcinoma cell lines (AsPC-1 and PANC-1), providing the first definitive evidence of air gene presence in human genome. In addition, we demonstrate that air expression is induced in the tumor cell lines by a naturally occurring ROS-inducing compound, phenethyl isothiocyanate (PEITC), a potential dietary cancer chemopreventive agent. Since PEITC inhibits NF-κB activation, air induction by this agent is consistent with the suppressive effect of NF-κB on air expression. This finding contributes a new clue to the role of NF-κB in regulating oxidative stress-induced pro-apoptotic genes and identifies a novel potential tool for enhancing pancreas cancer response to treatment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
