Supercritical CO2 (SC-CO2) extraction coupled with fractional separation of the extracts, has been used to concentrate the active principles of Ruta graveolens. Using 250 bar, 40 °C for the extraction process and cooling the first separator to −10 °C, an efficient extraction and fractionation was obtained. Different particle sizes of the vegetable matter were tested (from 0.20 to 0.80 mm); they indicated that the extraction process was faster when particle size was smaller; i.e., internal mass transfer controlled the process. Fractional separation allowed the selective elimination of cuticular waxes, that represented about 78% w/w of the total extract produced by SC-CO2processing. The extract collected in the second separator was concentrated in active compounds up to 87% w/w. For comparison purposes, extraction was also performed using an organic solvent: the extract obtained using ethyl-acetate, contained low percentages of active compounds, mainly due to the co-extraction of large quantities of waxes.

Concentration of Ruta graveolens active compounds using SC-CO2 extraction coupled with fractional separation

Baldino, Lucia;Adami, Renata;Reverchon, Ernesto
2018-01-01

Abstract

Supercritical CO2 (SC-CO2) extraction coupled with fractional separation of the extracts, has been used to concentrate the active principles of Ruta graveolens. Using 250 bar, 40 °C for the extraction process and cooling the first separator to −10 °C, an efficient extraction and fractionation was obtained. Different particle sizes of the vegetable matter were tested (from 0.20 to 0.80 mm); they indicated that the extraction process was faster when particle size was smaller; i.e., internal mass transfer controlled the process. Fractional separation allowed the selective elimination of cuticular waxes, that represented about 78% w/w of the total extract produced by SC-CO2processing. The extract collected in the second separator was concentrated in active compounds up to 87% w/w. For comparison purposes, extraction was also performed using an organic solvent: the extract obtained using ethyl-acetate, contained low percentages of active compounds, mainly due to the co-extraction of large quantities of waxes.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11386/4709341
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 32
  • ???jsp.display-item.citation.isi??? 31
social impact