A comparison between the transmembrane voltage time behaviour (TMV) of an excitable cell versus a non-excitable one is performed by using a three-dimensional lattice transport model. The hypothesis of a cell subjected to a typical electric field used in the Electrochemotherapy Treatment (ECT) is considered. In particular, the case of a single spherical cell exposed to a Pulsed Electric Field (PEF) of trapezoidal shape (i.e. 100s PEF of 70kV/m with a rise and fall time equal to 4s) is analysed. The excitable cell is modelled by means of a 3D electro-physical circuit implementing the compartmental model of a Normal Rat Kidney cell (NRK) whereas a comparable non-excitable cell is obtained in the hypothesis of absence of voltage controlled channels. The model is capable to spatially distinguish six different parts of the cell. The comparison of the time behaviour of the TMV at the anodic and cathodic region puts in evidence a weak influence of the excitability of the cell with respect to the considered ECT stress in terms of electroporation (EP). The principal differences appear in the time onset of the EP phenomenon and in the OFF phase of the input signal showing a potential influence if the repetition frequency is considered.

CIRCUITAL BASED ANALYSIS FOR THE EVALUATION OF THE ROLE OF THE CELL EXCITABILITY WITH RESPECT TO ELECTROCHEMOTHERAPY TREATMENT STRESS

CITRO, NUNZIANTE;LAMBERTI, PATRIZIA;TUCCI, Vincenzo;
2012

Abstract

A comparison between the transmembrane voltage time behaviour (TMV) of an excitable cell versus a non-excitable one is performed by using a three-dimensional lattice transport model. The hypothesis of a cell subjected to a typical electric field used in the Electrochemotherapy Treatment (ECT) is considered. In particular, the case of a single spherical cell exposed to a Pulsed Electric Field (PEF) of trapezoidal shape (i.e. 100s PEF of 70kV/m with a rise and fall time equal to 4s) is analysed. The excitable cell is modelled by means of a 3D electro-physical circuit implementing the compartmental model of a Normal Rat Kidney cell (NRK) whereas a comparable non-excitable cell is obtained in the hypothesis of absence of voltage controlled channels. The model is capable to spatially distinguish six different parts of the cell. The comparison of the time behaviour of the TMV at the anodic and cathodic region puts in evidence a weak influence of the excitability of the cell with respect to the considered ECT stress in terms of electroporation (EP). The principal differences appear in the time onset of the EP phenomenon and in the OFF phase of the input signal showing a potential influence if the repetition frequency is considered.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11386/3881828
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