In this study the purposely synthetized amphiphilic copolymer α,β-poly(N-2-hydroxyethyl)-DL- aspartamide – Diethylenetriamine - Polylactic acid (PHEA-DETA-PLA) was used to produce cationic nanoparticles for NABDs delivery. The preparative protocol is based on multiple emulsions assisted by ultrasonic energy technique, method previously developed for polyesters and polyacrylates [4] and thus successfully applied to achieve nanovectors of the amphiphilic copolymer α,β-poly(N-2-hydroxyethyl)-DL-aspartamide–polylactic acid (PHEA–PLA) [5]. Double stranded DNA (dsDNA) with 12833.4 g/mol as molecular weight and simulating Homo sapiens siRNA probe Luciferase, was used as model nucleic acid based drug. dsDNA was loaded by complexation phenomenon onto nanoparticles surface through suitable contact stage between cationic polymeric nanoparticles and dsDNA aqueous solution.
NANOPARTICLES BASED ON PHEA–DETA-PLA COPOLYMER FOR NABDs DELIVERY
BOCHICCHIO , SABRINA;DALMORO, ANNALISA;CAVALLARO, GENNARA;Sardo, Carla;LAMBERTI, Gaetano;BARBA, Anna Angela
2016
Abstract
In this study the purposely synthetized amphiphilic copolymer α,β-poly(N-2-hydroxyethyl)-DL- aspartamide – Diethylenetriamine - Polylactic acid (PHEA-DETA-PLA) was used to produce cationic nanoparticles for NABDs delivery. The preparative protocol is based on multiple emulsions assisted by ultrasonic energy technique, method previously developed for polyesters and polyacrylates [4] and thus successfully applied to achieve nanovectors of the amphiphilic copolymer α,β-poly(N-2-hydroxyethyl)-DL-aspartamide–polylactic acid (PHEA–PLA) [5]. Double stranded DNA (dsDNA) with 12833.4 g/mol as molecular weight and simulating Homo sapiens siRNA probe Luciferase, was used as model nucleic acid based drug. dsDNA was loaded by complexation phenomenon onto nanoparticles surface through suitable contact stage between cationic polymeric nanoparticles and dsDNA aqueous solution.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
