Short interfering RNAs (siRNAs) (1), represent an emerging paradigm for the treatment of many human diseases (2-3), however, owing to its hydrophilicity and negative charges, siRNA molecules are not readily taken up by cells and are susceptible to nuclease degradation (4). Thus, much effort has been placed in the development of siRNA delivery systems. Polyplexes, colloidal systems originated from the electrostatic interaction between negative nucleic acids and positive charged polymers, emerged as one of the most versatile systems for nucleic acid delivery (5). In this work, diethylenetriamine (DETA) molecules were grafted onto inulin backbone to generate a novel siRNA delivery system (Inu-DETA).
SIMPLE, BIOCOMPATIBLE AND COST-EFFECTIVE INULIN BASED siRNA DELIVERY SYSTEMS
Sardo C;Cavallaro G;
2014-01-01
Abstract
Short interfering RNAs (siRNAs) (1), represent an emerging paradigm for the treatment of many human diseases (2-3), however, owing to its hydrophilicity and negative charges, siRNA molecules are not readily taken up by cells and are susceptible to nuclease degradation (4). Thus, much effort has been placed in the development of siRNA delivery systems. Polyplexes, colloidal systems originated from the electrostatic interaction between negative nucleic acids and positive charged polymers, emerged as one of the most versatile systems for nucleic acid delivery (5). In this work, diethylenetriamine (DETA) molecules were grafted onto inulin backbone to generate a novel siRNA delivery system (Inu-DETA).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
