The remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications however, in several important cases, the interfacing of separation techniques with mass spectrometry still suffer of poor compatibility. Among different approaches, porous graphitic carbon (PGC) is a HPLC stationary phase which appear especially appropriate for small ions or neutral polar compounds scarcely retained by reversed phases1 nevertheless, in spite of its attractive peculiar characteristics, the use of porous graphitic carbon (PGC) never took off in standard environmental and bio-analytical labs. A possible reason for it lies in the complexity of its dual behavior2 . PGC, in fact, provides a mixture of interactions3 , both strongly hydrophobic and electronic4 , thus making difficult the predictions regarding its retention ability. Another possible reason for the “underuse” of this stationary phase is that many components of environmental or biological samples can be strongly adsorbed on it suddenly modifying the retention characteristic of the column5 . This might result in the need for an additional column washing and reconditioning procedure. Nevertheless, several challenging separations of ionic or highly polar and hydrophilic compounds can benefit of PGC retention abilities and its excellent selectivity6,7. The results of several studies devoted to outline general use guidelines for PGC in combination with ESIMS detection will be presented and the performances compared with those of ion chromatography (IC), classic reversed phase (RP) and hydrophilic interaction chromatography (HILIC).
Applications of Porous Graphitic Carbon in liquid chromatography – mass spectrometry
Carlo Crescenzi
;GRASSO, GIULIANA;
2017-01-01
Abstract
The remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications however, in several important cases, the interfacing of separation techniques with mass spectrometry still suffer of poor compatibility. Among different approaches, porous graphitic carbon (PGC) is a HPLC stationary phase which appear especially appropriate for small ions or neutral polar compounds scarcely retained by reversed phases1 nevertheless, in spite of its attractive peculiar characteristics, the use of porous graphitic carbon (PGC) never took off in standard environmental and bio-analytical labs. A possible reason for it lies in the complexity of its dual behavior2 . PGC, in fact, provides a mixture of interactions3 , both strongly hydrophobic and electronic4 , thus making difficult the predictions regarding its retention ability. Another possible reason for the “underuse” of this stationary phase is that many components of environmental or biological samples can be strongly adsorbed on it suddenly modifying the retention characteristic of the column5 . This might result in the need for an additional column washing and reconditioning procedure. Nevertheless, several challenging separations of ionic or highly polar and hydrophilic compounds can benefit of PGC retention abilities and its excellent selectivity6,7. The results of several studies devoted to outline general use guidelines for PGC in combination with ESIMS detection will be presented and the performances compared with those of ion chromatography (IC), classic reversed phase (RP) and hydrophilic interaction chromatography (HILIC).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
