Interactome analysis of bioactive molecules: optimization of a functional proteomics platform

The identification of natural products (NPs) target proteins is pivotal to understand their mechanism of action, in order to develop molecular probes and/or potential drugs. In the last 15 years, affinity chromatography-coupled to mass spectrometry (AP-MS) has been the top-choice technique in the Drug Target Deconvolution field, having brought brilliant results in the targetome profiling of a multitude of bioactive compounds. Unfortunately, since a chemical modification of the molecule to be investigated is mandatory, AP MS is not suitable for compounds that do not exhibit properly reactive structural feature. .. [edited by the Author]