Neutrophils, the most abundant circulating leukocyte population, are principally involved in pathogen clearance through three major mechanisms: phagocytosis, degranulation, and release of neutrophil extracellular traps. Along with these roles, neutrophils can promote or suppress tumor growth and metastatic processes. These different immunological functions reflect a great heterogeneity of maturation and activation status, as at least two main circulating populations can be identified: low-density granulocytes and normal-density neutrophils. In this chapter, we provide a detailed protocol for multiparametric flow cytometry immunophenotyping of circulating and bone marrow neutrophil subsets, as well as recommendations for protocol optimization, troubleshooting, and clinical sample analysis.
Neutrophil Subset Immunophenotyping
Gorrese M.;Selleri C.;Giudice V.
2027
Abstract
Neutrophils, the most abundant circulating leukocyte population, are principally involved in pathogen clearance through three major mechanisms: phagocytosis, degranulation, and release of neutrophil extracellular traps. Along with these roles, neutrophils can promote or suppress tumor growth and metastatic processes. These different immunological functions reflect a great heterogeneity of maturation and activation status, as at least two main circulating populations can be identified: low-density granulocytes and normal-density neutrophils. In this chapter, we provide a detailed protocol for multiparametric flow cytometry immunophenotyping of circulating and bone marrow neutrophil subsets, as well as recommendations for protocol optimization, troubleshooting, and clinical sample analysis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
