The major flavonoids in the white florets of chamomile (Chamomilla recutita [L.] Rauschert) were rapidly purified using a combination of polyamide solid-phase extraction and preparative HPLC. From the combined LC/MS, LC/MS/MS, and NMR data the apigenin glucosides were identified as apigenin 7-O-glucoside (Ap-7-Glc), Ap-7-(6''-malonyl-Glc), Ap-7-(6''-acetyl-Glc), Ap-7-(6''- caffeoyl-Glc), Ap-7-(4-''acetyl-Glc), Ap-7-(4''-acetyl,6-''malonyl-Glc), and a partially characterised apigenin-7-(mono-acetyl/monomalonylglucoside) isomer. Malonyl and caffeoyl derivatives of Ap-7-Glc have not previously been identified in chamomile. The two mono-acetyl/mono-malonyl flavonoids have not previously been reported in any plant species. These acylated glucosides are unstable and degrade to form acetylated compounds or Ap-7-Glc. The degradation products formed are dependent on the extraction and storage conditions, i.e. temperature, pH and solvent.
Titolo: | Isolation, identification and stability of acylated derivatives of apigenin 7-O-glucoside from chamomile (Chamomilla recutita [L.] Rauschert). | |
Autori: | ||
Data di pubblicazione: | 2004 | |
Rivista: | ||
Abstract: | The major flavonoids in the white florets of chamomile (Chamomilla recutita [L.] Rauschert) were rapidly purified using a combination of polyamide solid-phase extraction and preparative HPLC. From the combined LC/MS, LC/MS/MS, and NMR data the apigenin glucosides were identified as apigenin 7-O-glucoside (Ap-7-Glc), Ap-7-(6''-malonyl-Glc), Ap-7-(6''-acetyl-Glc), Ap-7-(6''- caffeoyl-Glc), Ap-7-(4-''acetyl-Glc), Ap-7-(4''-acetyl,6-''malonyl-Glc), and a partially characterised apigenin-7-(mono-acetyl/monomalonylglucoside) isomer. Malonyl and caffeoyl derivatives of Ap-7-Glc have not previously been identified in chamomile. The two mono-acetyl/mono-malonyl flavonoids have not previously been reported in any plant species. These acylated glucosides are unstable and degrade to form acetylated compounds or Ap-7-Glc. The degradation products formed are dependent on the extraction and storage conditions, i.e. temperature, pH and solvent. | |
Handle: | http://hdl.handle.net/11386/1065094 | |
Appare nelle tipologie: | 1.1.2 Articolo su rivista con ISSN |