Tree-of-heaven (Ailanthus altissima Swingle) was evaluated for its cytotoxic and antiproliferative activities by a bioassay-oriented study. Cytotoxicity observed in HeLa cells was time-dependent; the treatment with 10 μg/ mL of the root chloroform extract reduced cell viability by 56% at 24 h and 29% at 48 h of exposure, whereas no effect was recorded in the controls. Significant effects were observed also for chromatographic fractions and the pure isolated alkaloid 1-methoxy-canthin-6-one. After 72 h of incubation cell viability was less than 10% for all treatments. A possible apoptotic effect was evaluated by monitoring the presence of hypodiploid elements in HeLa cells as well as in SAOS, U87MG and U-937 tumor cell lines. The cells incubated for different times with the active extract, fraction and pure alkaloid isolated from A. altissima showed a remarkable increase in the apoptosis.

Antiproliferative effects of tree-of-heaven (Ailanthus altissima Swingle)

DE FEO, Vincenzo;DE MARTINO, LAURA;PIZZA, Cosimo;FRANCESCHELLI, Silvia;PASCALE, Maria
2005-01-01

Abstract

Tree-of-heaven (Ailanthus altissima Swingle) was evaluated for its cytotoxic and antiproliferative activities by a bioassay-oriented study. Cytotoxicity observed in HeLa cells was time-dependent; the treatment with 10 μg/ mL of the root chloroform extract reduced cell viability by 56% at 24 h and 29% at 48 h of exposure, whereas no effect was recorded in the controls. Significant effects were observed also for chromatographic fractions and the pure isolated alkaloid 1-methoxy-canthin-6-one. After 72 h of incubation cell viability was less than 10% for all treatments. A possible apoptotic effect was evaluated by monitoring the presence of hypodiploid elements in HeLa cells as well as in SAOS, U87MG and U-937 tumor cell lines. The cells incubated for different times with the active extract, fraction and pure alkaloid isolated from A. altissima showed a remarkable increase in the apoptosis.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11386/1847103
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