Brassica napus cultivars resistant to Leptosphaeria maculans have been widely used to control the blackleg disease. The B. napus cv. Surpass 400, with major gene resistance derived from B. rapa ssp. sylvestris, was inoc- ulated either with a virulent or an avirulent isolate of L. maculans, and hanges in the cotyledon protein patterns were studied by proteomic analysis. The two dimensional (2D) protein gels indicated a plant response quantitatively and qualitatively different, when the compatible and the incompatible interactions were compared. Many differential spots were found in the B. napus proteome subjected to trypsin digestion and analysed by MALDI-TOF mass spectrometry. Peptide mass fingerprinting (PMF), together with data available in the literature, allowed the identification of many proteins involved in stress-related and antioxidant responses, CO2 fixation, nutrient metabolism, etc. The putative role of some of the identified factors in plant susceptibility or resistance to blackleg disease is discussed.

Proteomic analysis of the interaction between Brassica napus cv. surpass 400 and virulent or avirulent isolates of Leptosphaeria maculans

CAVALLO, Pierpaolo;
2010

Abstract

Brassica napus cultivars resistant to Leptosphaeria maculans have been widely used to control the blackleg disease. The B. napus cv. Surpass 400, with major gene resistance derived from B. rapa ssp. sylvestris, was inoc- ulated either with a virulent or an avirulent isolate of L. maculans, and hanges in the cotyledon protein patterns were studied by proteomic analysis. The two dimensional (2D) protein gels indicated a plant response quantitatively and qualitatively different, when the compatible and the incompatible interactions were compared. Many differential spots were found in the B. napus proteome subjected to trypsin digestion and analysed by MALDI-TOF mass spectrometry. Peptide mass fingerprinting (PMF), together with data available in the literature, allowed the identification of many proteins involved in stress-related and antioxidant responses, CO2 fixation, nutrient metabolism, etc. The putative role of some of the identified factors in plant susceptibility or resistance to blackleg disease is discussed.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11386/2600415
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 4
social impact