In a previous paper the immobilization of yeast (S. cerevisiae) by adhesion to tuff granules was described as a convenient alternative method to gel entrapment. In fact, immobilization by adhesion offers advantages over gel entrapment because it is simple and free from internal diffusion limitations. Since in microbial cell adhesion a fundamental role is played by the substratum, i.e.. the solid surface to which the microorganism attach, a systematic study was undertaken to examine the possibility of using different materials as substrate for yeast cells in a continuous operational mode. Thus, polymeric hydrogels belonging to the same class of polymeric matrices obtained by radiation-induced polymerization and successfully used to immobilized cells, enzymes and antibodies, were chosen. Eight of such polymeric matrices with different hydrophilicity were synthetized and assayed as substrata for yeast cell operating in continuous alcoholic fermentation.
Adhesion of yeast cells (Saccharomyces cerevisiae) to synthetic polimeric carriers
PARASCANDOLA, Palma;
1989-01-01
Abstract
In a previous paper the immobilization of yeast (S. cerevisiae) by adhesion to tuff granules was described as a convenient alternative method to gel entrapment. In fact, immobilization by adhesion offers advantages over gel entrapment because it is simple and free from internal diffusion limitations. Since in microbial cell adhesion a fundamental role is played by the substratum, i.e.. the solid surface to which the microorganism attach, a systematic study was undertaken to examine the possibility of using different materials as substrate for yeast cells in a continuous operational mode. Thus, polymeric hydrogels belonging to the same class of polymeric matrices obtained by radiation-induced polymerization and successfully used to immobilized cells, enzymes and antibodies, were chosen. Eight of such polymeric matrices with different hydrophilicity were synthetized and assayed as substrata for yeast cell operating in continuous alcoholic fermentation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.