The presence of rhodanese activity has been investigated in two strains of termophilic eubacteria and two strains of extremophiles. Bacillus acidocaldarius, a thermoacidophilic eubacterium, showed the highest levels of enzyme activity. Whole cells previously subjected to one cycle of freeze-thawing were immobilized by entrapment in the polysaccharide matrices Ca-alginate, k-carrageenan, and chitosan, and in an insolubilized gelatine gel. The results obtained with the different immobilization in terms of activity yield, possibility of regeneration and operative stability were evaluated with the aim of setting up a continuous system. This was achieved with a system consisting of Bacillus acidocaldarius cells entrapped in an insolubilised gelatin matrix. The latter, in the form of a thin membrane, was employed in a custom-conceived reactor operating as a plug-flow reactor.
Immobilization of Bacillus acidocaldarius whole-cell rhodanese in polysaccharide and insolubilized gelatin gels"
PARASCANDOLA, Palma
1996-01-01
Abstract
The presence of rhodanese activity has been investigated in two strains of termophilic eubacteria and two strains of extremophiles. Bacillus acidocaldarius, a thermoacidophilic eubacterium, showed the highest levels of enzyme activity. Whole cells previously subjected to one cycle of freeze-thawing were immobilized by entrapment in the polysaccharide matrices Ca-alginate, k-carrageenan, and chitosan, and in an insolubilized gelatine gel. The results obtained with the different immobilization in terms of activity yield, possibility of regeneration and operative stability were evaluated with the aim of setting up a continuous system. This was achieved with a system consisting of Bacillus acidocaldarius cells entrapped in an insolubilised gelatin matrix. The latter, in the form of a thin membrane, was employed in a custom-conceived reactor operating as a plug-flow reactor.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
