Free and gelatin immobilized yeast cells from two different strains were analyzed by means of SDS-PAGE as regards: i) the glycosylation levels of external invertase before and after treatment with endo-beta-N-acetylglycosaminidase (Endo H), ii) the pattern of mannoproteins released from the cell wall after extraction with SDS, beta-mercaptoethanol and Zymoliase digestion. The results obtain suggest that the composition and structure of the cell wall is modified when cell growth takes place in the state of immobilization.
Gelatin immobilized growing yeast cells: changes in the glycosylation level of external invertase and cell wall composition
PARASCANDOLA, Palma
1996-01-01
Abstract
Free and gelatin immobilized yeast cells from two different strains were analyzed by means of SDS-PAGE as regards: i) the glycosylation levels of external invertase before and after treatment with endo-beta-N-acetylglycosaminidase (Endo H), ii) the pattern of mannoproteins released from the cell wall after extraction with SDS, beta-mercaptoethanol and Zymoliase digestion. The results obtain suggest that the composition and structure of the cell wall is modified when cell growth takes place in the state of immobilization.File in questo prodotto:
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