Seven new polyhydroxylated sulfated sterols (I-VII), all possessing 3α,21-disulfoxy-4α-hydroxy substituents and the A/B cis ring junction, were isolated from the ophiuroid O. superba, collected at Okinawa, Japan. Four sterols possessed identical nuclei (i.e., 3α-sulfoxy-4α-hydroxy) but differed in the side chain. Two possessed 1 more OH group in the nucleus located at C-2β, and one had the extra OH group at C-5B. Their general structure was deduced from spectral data (1H and 13C NMR and fast-atom-bombardment mass spectra), and the stereochem. of some of them was detd. by correlating their resp. spectral data with those of synthetic sterols.