L929 and WEHI tumor cell lines were genetically modified to constitutively express the Saccharomyces cerevisiae Ole 1 gene, coding for the delta 9-desaturase enzyme. These cells exhibit an increased ratio of monounsaturated fatty acids in their membrane phospholipids paralleled by an overall decrease in the membrane molecular order and a highly increased tumor necrosis factor-alpha (TNF) sensitivity. The TNF-alpha signaling cascade involves events, like receptor clustering and cleavage of membrane constituent lipid molecules by phospholipases, which are influenced by the physical state of cellular membranes. We discuss the possible involvement of non-bilayer forming lipids in the control of signaling mechanisms leading to TNF cytotoxicity.
Highly increased TNF sensitivity of tumor cells expresing the yeast Δ9-desaturase gene.
MARESCA, Bruno;
1997-01-01
Abstract
L929 and WEHI tumor cell lines were genetically modified to constitutively express the Saccharomyces cerevisiae Ole 1 gene, coding for the delta 9-desaturase enzyme. These cells exhibit an increased ratio of monounsaturated fatty acids in their membrane phospholipids paralleled by an overall decrease in the membrane molecular order and a highly increased tumor necrosis factor-alpha (TNF) sensitivity. The TNF-alpha signaling cascade involves events, like receptor clustering and cleavage of membrane constituent lipid molecules by phospholipases, which are influenced by the physical state of cellular membranes. We discuss the possible involvement of non-bilayer forming lipids in the control of signaling mechanisms leading to TNF cytotoxicity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
