The composition and physical state of membrane lipids determine the dynamic nature of membranes, which in turn, could directly be linked to the activity of various membrane-associated cellular functions. To better understand the molecular basis of different membrane-related phenomena we established a novel strategy to alter unsaturation of mammalian cell membranes with an identical genetic background. We transfected L929 mouse fibroblastoid cells with DNA constructs containing the Δ9-fatty acid desaturase gene (Ole1) ofS. cerevisiaeunder the control of desaturase promoters derived either from wild type or mutant strains of the dimorphic fungusH. capsulatum.
Modulation of lipid unsaturation and membrane fluidity in mammalian cells by transfection of an up-regulated Δ9-desaturase promoter.
MARESCA, Bruno
1997-01-01
Abstract
The composition and physical state of membrane lipids determine the dynamic nature of membranes, which in turn, could directly be linked to the activity of various membrane-associated cellular functions. To better understand the molecular basis of different membrane-related phenomena we established a novel strategy to alter unsaturation of mammalian cell membranes with an identical genetic background. We transfected L929 mouse fibroblastoid cells with DNA constructs containing the Δ9-fatty acid desaturase gene (Ole1) ofS. cerevisiaeunder the control of desaturase promoters derived either from wild type or mutant strains of the dimorphic fungusH. capsulatum.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
