A validated anal. method for the quant. detn. of seven chem. markers occurring in a hydroalcoholic ext. of Vitex agnus-castus fruits by liq. chromatog. electrospray triple quadrupole tandem mass spectrometry (LC/ESI/(QqQ)MSMS) is reported. To carry out a comparative study, five com. food supplements corresponding to hydroalcoholic exts. of V. agnus-castus fruits were analyzed under the same chromatog. conditions of the crude ext. Principal component anal. (PCA), based only on the variation of the amt. of the seven chem. markers, was applied in order to find similarities between the hydroalcoholic ext. and the food supplements. A second PCA anal. was carried out considering the whole spectroscopic data deriving from liq. chromatog. electrospray linear ion trap mass spectrometry (LC/ESI/(LIT)MS) anal. High similarity between the two PCA was obsd., showing the possibility to select one of these two approaches for future applications in the field of comparative anal. of food supplements and quality control procedures.
Liquid chromatography tandem mass spectrometry determination of chemical markers and principal component analysis of Vitex agnus-castus L. fruits (Verbenaceae) and derived food supplements
MARI, ANGELA;MONTORO, Paola;PIZZA, Cosimo;PIACENTE, Sonia
2012-01-01
Abstract
A validated anal. method for the quant. detn. of seven chem. markers occurring in a hydroalcoholic ext. of Vitex agnus-castus fruits by liq. chromatog. electrospray triple quadrupole tandem mass spectrometry (LC/ESI/(QqQ)MSMS) is reported. To carry out a comparative study, five com. food supplements corresponding to hydroalcoholic exts. of V. agnus-castus fruits were analyzed under the same chromatog. conditions of the crude ext. Principal component anal. (PCA), based only on the variation of the amt. of the seven chem. markers, was applied in order to find similarities between the hydroalcoholic ext. and the food supplements. A second PCA anal. was carried out considering the whole spectroscopic data deriving from liq. chromatog. electrospray linear ion trap mass spectrometry (LC/ESI/(LIT)MS) anal. High similarity between the two PCA was obsd., showing the possibility to select one of these two approaches for future applications in the field of comparative anal. of food supplements and quality control procedures.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
