Galacturonic acid is a monosaccharide obtained by pectin hydrolysis and a suitable substrate to produce bioethanol by fermentation. This paper focuses on quantification of citrus pectin hydrolysis to galacturonic acid and provides new, reliable kinetic parameters for the Michaelis-Menten equation when the well-known, commercial Pectinex Ultra SP-L is employed as enzyme. They are: rmax=1.10 g/ (L min), Km=10.42g/L and KIGA=10.05g/L, as obtained with a great accuracy by a non-linear regression method and confirmed by the three classical linearization procedures (Lineweaver-Burk, Langmuir and Eadie-Hofstee). The quantification of product inhibition has been achieved, with its inclusion in the rate equation. A batch reactor model yields a perfect agreement between predictions and experiments, even under conditions different from those on which the parameters had been determined by regression

Optimal kinetic parameters and batch modeling for pectin hydrolysis to galacturonic acid with Pectinex Ultra SP-L enzyme

GHORBANPOUR KHAMSEH, ALI' ASGHAR;MICCIO, Michele;FERRARI, Giovanna
2013

Abstract

Galacturonic acid is a monosaccharide obtained by pectin hydrolysis and a suitable substrate to produce bioethanol by fermentation. This paper focuses on quantification of citrus pectin hydrolysis to galacturonic acid and provides new, reliable kinetic parameters for the Michaelis-Menten equation when the well-known, commercial Pectinex Ultra SP-L is employed as enzyme. They are: rmax=1.10 g/ (L min), Km=10.42g/L and KIGA=10.05g/L, as obtained with a great accuracy by a non-linear regression method and confirmed by the three classical linearization procedures (Lineweaver-Burk, Langmuir and Eadie-Hofstee). The quantification of product inhibition has been achieved, with its inclusion in the rate equation. A batch reactor model yields a perfect agreement between predictions and experiments, even under conditions different from those on which the parameters had been determined by regression
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11386/3880278
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact