Galacturonic acid is a monosaccharide obtained by pectin hydrolysis and a suitable substrate to produce bioethanol by fermentation. This paper focuses on quantification of citrus pectin hydrolysis to galacturonic acid and provides new, reliable kinetic parameters for the Michaelis-Menten equation when the well-known, commercial Pectinex Ultra SP-L is employed as enzyme. They are: rmax=1.10 g/ (L min), Km=10.42g/L and KIGA=10.05g/L, as obtained with a great accuracy by a non-linear regression method and confirmed by the three classical linearization procedures (Lineweaver-Burk, Langmuir and Eadie-Hofstee). The quantification of product inhibition has been achieved, with its inclusion in the rate equation. A batch reactor model yields a perfect agreement between predictions and experiments, even under conditions different from those on which the parameters had been determined by regression
Optimal kinetic parameters and batch modeling for pectin hydrolysis to galacturonic acid with Pectinex Ultra SP-L enzyme
GHORBANPOUR KHAMSEH, ALI' ASGHAR;MICCIO, Michele;FERRARI, Giovanna
2013
Abstract
Galacturonic acid is a monosaccharide obtained by pectin hydrolysis and a suitable substrate to produce bioethanol by fermentation. This paper focuses on quantification of citrus pectin hydrolysis to galacturonic acid and provides new, reliable kinetic parameters for the Michaelis-Menten equation when the well-known, commercial Pectinex Ultra SP-L is employed as enzyme. They are: rmax=1.10 g/ (L min), Km=10.42g/L and KIGA=10.05g/L, as obtained with a great accuracy by a non-linear regression method and confirmed by the three classical linearization procedures (Lineweaver-Burk, Langmuir and Eadie-Hofstee). The quantification of product inhibition has been achieved, with its inclusion in the rate equation. A batch reactor model yields a perfect agreement between predictions and experiments, even under conditions different from those on which the parameters had been determined by regressionI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
