β-Sitosterol, lupeol and daucosterol have been isolated from ethanolic extract of Randia dumetorum bark. β-Sitosterol, Lupeol and Daucosterol have been isolated for the first time from the Randia dumetorum. β Sitosterol and Daucosterol were identified by complete interpretation of 1H NMR and 13C NMR spectra using 2D NMR (HSQC, HMBC, DQF-COSY) experiments and Lupeol by direct comparison with authentic sample. The ethanolic extract is subjected to cytotoxic activity against A549 cell (adenocarcinomic human alveolar basal epithelial cells), BE (2) C (Neuroblastonoma Cell Line), Hela, MDA-MB-231 (breast cancer cell line), SK MEL-2 (Human Skin Melanoma Cell) and U87MG cell (Human Neuronale Gliblostoma astrozytom Cell Line) which showed most effective cytotoxic activity at 400 μg/mL concentration on MDA-MB-231 (Breast Cancer Cell Line).
CHEMICAL INVESTIGATION AND IN VITRO CYTOTOXIC ACTIVITY OF RANDIA DUMETORUM LAMK. BARK
AQUINO, Rita Patrizia;MENCHERINI, TERESA;
2012-01-01
Abstract
β-Sitosterol, lupeol and daucosterol have been isolated from ethanolic extract of Randia dumetorum bark. β-Sitosterol, Lupeol and Daucosterol have been isolated for the first time from the Randia dumetorum. β Sitosterol and Daucosterol were identified by complete interpretation of 1H NMR and 13C NMR spectra using 2D NMR (HSQC, HMBC, DQF-COSY) experiments and Lupeol by direct comparison with authentic sample. The ethanolic extract is subjected to cytotoxic activity against A549 cell (adenocarcinomic human alveolar basal epithelial cells), BE (2) C (Neuroblastonoma Cell Line), Hela, MDA-MB-231 (breast cancer cell line), SK MEL-2 (Human Skin Melanoma Cell) and U87MG cell (Human Neuronale Gliblostoma astrozytom Cell Line) which showed most effective cytotoxic activity at 400 μg/mL concentration on MDA-MB-231 (Breast Cancer Cell Line).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
