The discovery of inhibitors of methyl- and acetyl-binding domains has provided evidence for the “druggability” of epigenetic effector molecules. Using a library of biotin-tagged analogs we screened a protein domain microarray of methyl-lysine effector molecules to rapidly detect compounds with novel binding profiles - either improved or loosened specificity. Using this approach, we identified compounds that acquired novel interactions with Tudor domain-and MBT domain-containing proteins.
|Titolo:||Sympathy for the methyl: a library-on-library screening approach to identify small-molecule ligands of methyl-lysine reader proteins|
|Data di pubblicazione:||2017|
|Appare nelle tipologie:||4.1 Contributi in Atti di convegno|