Hydrogen sulfide (H2S) is an important biological messenger, which recently joined nitric oxide (NO) and carbon monoxide (CO) as an endogenously produced gasotransmitter. This discovery resulted in a renewed interest towards H2S and, in particular, in its reactivity with biological and bioinorganic targets. However, in the scientific literature studies exploring the reactivity of H2S/HS– with non-heme proteins are rare. In this work, we studied the interaction of HS– with the natural metalloprotein Cu-azurin and its cobalt variant via UV-Vis, fluorescence, and EPR spectroscopy, as well as X-ray diffraction. Interaction of HS– significantly enhances the fluorescence emission of fluorescently labelled copper azurin in buffered solutions and at physiological pH. In contrast, fluorescently labelled cobalt azurin undergoes a fluorescence quenching in the presence of HS– in line with the changes observed in the absorption spectrum of the construct. The results highlight the potential of azurin for implementation in a fast and simple HS– sensor.

Azurin and HS–: Towards Implementation of a Sensor for HS– Detection

Strianese, Maria;Pellecchia, Claudio
2019

Abstract

Hydrogen sulfide (H2S) is an important biological messenger, which recently joined nitric oxide (NO) and carbon monoxide (CO) as an endogenously produced gasotransmitter. This discovery resulted in a renewed interest towards H2S and, in particular, in its reactivity with biological and bioinorganic targets. However, in the scientific literature studies exploring the reactivity of H2S/HS– with non-heme proteins are rare. In this work, we studied the interaction of HS– with the natural metalloprotein Cu-azurin and its cobalt variant via UV-Vis, fluorescence, and EPR spectroscopy, as well as X-ray diffraction. Interaction of HS– significantly enhances the fluorescence emission of fluorescently labelled copper azurin in buffered solutions and at physiological pH. In contrast, fluorescently labelled cobalt azurin undergoes a fluorescence quenching in the presence of HS– in line with the changes observed in the absorption spectrum of the construct. The results highlight the potential of azurin for implementation in a fast and simple HS– sensor.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11386/4720905
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