Intestinal epithelial cells play a pivotal role in maintaining intestinal homeostasis. Diﬀerent noxious agents can damage the intestinal epithelial integrity. This damage is also associated with anticancer therapies resulting in reactive oxygen species (ROS) and pro-inﬂammatory factors overproduction, also at gastrointestinal level. Gastrointestinal mucositis is a frequent and severe side eﬀect of chemotherapy and radiotherapy in cancer patients, aﬀecting approximately 50 to 80% of patients [1,2]. Currently, no eﬀective treatment exists for chemotherapy-induced mucositis, prompting the need to develop an anti-mucositis agents for use in clinics. Our study focused on the eﬀect of Aloe barbadensis and Punica granatum combination in intestinal epithelial cells (IEC-6) during oxidative stress and inﬂammatory conditions. Oxidative stress and inﬂammatory mediators such as ROS release, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), heme oxygenase 1 (HO-1) and NAD(P)H Quinone Dehydrogenase 1 (NQO1) expression, were evaluated by cytoﬂuorimetric tecniques. Tumor necrosis factor-α (TNF-α) levels were evaluated by ELISA assay. Aloe barbadensis inhibited ROS production both during oxidative stress and in inﬂammatory conditions as well as COX-2 and iNOS expression, at all tested concentrations, during inﬂammation in IEC-6. In the same experimental conditions, Aloe barbadensis also increased the cytoprotective enzymes HO-1 and NQO1 expression and reduced TNF-α release. The treatment of IEC-6 with Aloe barbadensis plus Punica granatum (9:1 ratio) signiﬁcantly increases the activity of Aloe barbadensis alone. Our results indicate that Aloe barbadensis and Punica granatum combination could be useful to reduce the oxidative stress and inﬂammatory-mediated complications, also associated to chemotherapy and radiotherapy, at intestinal level. References  Peterson DE, Bensadoun RJ, Roila F, Group EGW. Management of oral and gastrointestinal mucositis: ESMO Clinical Practice Guidelines. Ann Oncol 2011; 22 (Suppl 6): vi78–84  Rosenthal DI, Trotti A. Strategies for managing radiation-induced mucositis in head and neck cancer. Semin Radiat Oncol 2009; 19: 29–34
Rapa, SF;Pepe, G;Cianciarulo, D;Merciai, F;Salviati, E;Sommella, E;Autore, G;Campiglia, P;Marzocco, S
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