A microfluidic based multiplex fluorescence signal readout system, able to fast, simply and accurately detect low concentrations of microRNA (miRNA) in small sample volumes is presented. Our ap-proach is able to simultaneously process several different miRNA types and moreover to measure their absolute amounts in a continuous microfluidic flow, without complex pre-treatments as PCR amplification steps. We additionally investigated the tendency of the utilized barcoded microgel parti-cles to be aligned, compared to rigid polystyrene particles. An improved 3D alignment has been ob-tained, according to the higher viscoelastic migration to the centreline due to the elasticity of the soft microgel particles.
Microgel-barcode readout for miRNA quantification in microfluidic flow
Rossi D.;
2016-01-01
Abstract
A microfluidic based multiplex fluorescence signal readout system, able to fast, simply and accurately detect low concentrations of microRNA (miRNA) in small sample volumes is presented. Our ap-proach is able to simultaneously process several different miRNA types and moreover to measure their absolute amounts in a continuous microfluidic flow, without complex pre-treatments as PCR amplification steps. We additionally investigated the tendency of the utilized barcoded microgel parti-cles to be aligned, compared to rigid polystyrene particles. An improved 3D alignment has been ob-tained, according to the higher viscoelastic migration to the centreline due to the elasticity of the soft microgel particles.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
