During a survey performed in opium felds grown at Acharya Narendra Deva University of Agriculture & Technology (ANDUAT), Kumarganj, Ayodhya, Uttar Pradesh, India in March 2021, symptoms of phyllody and stunting were observed on opium plants. The association of phytoplasmas was confrmed in all the symptomatic opium samples by using PCR of 16S rRNA and secA genes sequences. Association of a ‘Candidatus Phytoplasma australasia’ related strain was confrmed by 16S rRNA and secA gene sequence comparison analysis. Virtual RFLP analysis of ~1.25 kb product of 16S rRNA gene sequences using the iPhyClassifer tool further classifed the opium phytoplasma isolate into the 16SrII-D subgroup. This is the frst report on identifcation of a phytoplasma disease in opium in world.
First report of ‘Candidatus Phytoplasma australasia’ related strain associated with opium poppy phyllody and stunting in India
C. Marcone;
2023-01-01
Abstract
During a survey performed in opium felds grown at Acharya Narendra Deva University of Agriculture & Technology (ANDUAT), Kumarganj, Ayodhya, Uttar Pradesh, India in March 2021, symptoms of phyllody and stunting were observed on opium plants. The association of phytoplasmas was confrmed in all the symptomatic opium samples by using PCR of 16S rRNA and secA genes sequences. Association of a ‘Candidatus Phytoplasma australasia’ related strain was confrmed by 16S rRNA and secA gene sequence comparison analysis. Virtual RFLP analysis of ~1.25 kb product of 16S rRNA gene sequences using the iPhyClassifer tool further classifed the opium phytoplasma isolate into the 16SrII-D subgroup. This is the frst report on identifcation of a phytoplasma disease in opium in world.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
