: Callitris glaucophylla Joy Thomps. & L.A.S. Johnson is a coniferous forest species of the Cupressaceae family native to Australia. This species is rich in essential oils (EOs) but few studies about variability and biological activity of these EOs are available in the literature. The purpose of this study was to evaluate the variability of production of C. glaucophylla EOs in relation to the different plant parts (needles, cones and stems) and to investigate their antioxidant, anti-enzymatic and herbicidal properties. EOs were obtained by hydro distillation and analyzed by GC and GC-MS. The antioxidant potential of EOs was assessed by ABTS, FRAP and DPPH assays, their phytotoxic activities were evaluated against germination and shoots and radical growth of Sinapis arvensis, Trifolium campestre, Lepidium sativum and Lolium rigidum. The EOs were evaluated for their possible anti-enzymatic effects with spectrophotometric assay. EOs resulted rich in monoterpenes hydrocarbons (61.04-77.82 %) and oxygenated monoterpenes (19.52-25.26 %). The main compounds were α-pinene as major compound in all plant parts (36.99-59.84 %), 1,8-cineole (19.88 % in stems) and limonene (18.94 % in needles). Herbicidal assays showed that all EOs have remarkable and significant phytotoxicity towards germination, roots, and aerial parts growth of the tested plants, depending on the EO, the doses and tested species. The EOs showed significant free radical scavenging potential and resulted more active against cholinesterases than α-glucosidase and α-amylase. The data obtained constitute an important contribution in selecting and valorizing appropriate forestry tree biomass as sources of antioxidant and phytotoxic molecules for sustainable application in food preservation and weeds control. The activities against the tested enzymes confirmed a possible use of these EOs as natural pesticides.
Phytochemical study on the essential oils of Callitris glaucophylla Joy Thomps. & L.A.S. Johnson, and assessment of their antioxidant, anti-enzymatic and allelopathic effects
Polito, Flavio;Caputo, Lucia
;De Feo, Vincenzo
2024-01-01
Abstract
: Callitris glaucophylla Joy Thomps. & L.A.S. Johnson is a coniferous forest species of the Cupressaceae family native to Australia. This species is rich in essential oils (EOs) but few studies about variability and biological activity of these EOs are available in the literature. The purpose of this study was to evaluate the variability of production of C. glaucophylla EOs in relation to the different plant parts (needles, cones and stems) and to investigate their antioxidant, anti-enzymatic and herbicidal properties. EOs were obtained by hydro distillation and analyzed by GC and GC-MS. The antioxidant potential of EOs was assessed by ABTS, FRAP and DPPH assays, their phytotoxic activities were evaluated against germination and shoots and radical growth of Sinapis arvensis, Trifolium campestre, Lepidium sativum and Lolium rigidum. The EOs were evaluated for their possible anti-enzymatic effects with spectrophotometric assay. EOs resulted rich in monoterpenes hydrocarbons (61.04-77.82 %) and oxygenated monoterpenes (19.52-25.26 %). The main compounds were α-pinene as major compound in all plant parts (36.99-59.84 %), 1,8-cineole (19.88 % in stems) and limonene (18.94 % in needles). Herbicidal assays showed that all EOs have remarkable and significant phytotoxicity towards germination, roots, and aerial parts growth of the tested plants, depending on the EO, the doses and tested species. The EOs showed significant free radical scavenging potential and resulted more active against cholinesterases than α-glucosidase and α-amylase. The data obtained constitute an important contribution in selecting and valorizing appropriate forestry tree biomass as sources of antioxidant and phytotoxic molecules for sustainable application in food preservation and weeds control. The activities against the tested enzymes confirmed a possible use of these EOs as natural pesticides.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
