The nuclear receptor estrogen receptor 2 (ESR2, ERβ) modulates cancer cell proliferation and tumor growth, exerting an oncosuppressive role in breast cancer (BC). Interaction proteomics by tandem affinity purification coupled to mass spectrometry was previously applied in BC cells to identify proteins acting in concert with ERβ to control key cellular functions, including gene transcription, RNA splicing and post-transcriptional mRNA regulation. These studies revealed an involvement of RNA in ERβ interactome assembly and functions. By applying native protein complex purification followed by nano LC-MS/MS before and after in vitro RNA removal, we identified here a large set of nuclear ERβ-interactors, including a sizeable fraction associating with the receptor via RNA bridging. Combining label-free quantitation and GO analysis revealed several cellular functions involving the nuclear interactome mapped here. The data presented will be useful for studies aiming at elucidating the role of ERβ, nuclear RNAs and the other proteins identified here in BC and other cell types.

Quantitative mapping of RNA-mediated nuclear Estrogen Receptor β interactome in human breast cancer cells

Giurato, Giorgio
Investigation
;
Nassa, Giovanni
Investigation
;
Salvati, Annamaria
Investigation
;
Alexandrova, Elena
Investigation
;
Rizzo, Francesca
Investigation
;
Weisz, Alessandro
Supervision
;
Tarallo,Roberta
Supervision
2018-01-01

Abstract

The nuclear receptor estrogen receptor 2 (ESR2, ERβ) modulates cancer cell proliferation and tumor growth, exerting an oncosuppressive role in breast cancer (BC). Interaction proteomics by tandem affinity purification coupled to mass spectrometry was previously applied in BC cells to identify proteins acting in concert with ERβ to control key cellular functions, including gene transcription, RNA splicing and post-transcriptional mRNA regulation. These studies revealed an involvement of RNA in ERβ interactome assembly and functions. By applying native protein complex purification followed by nano LC-MS/MS before and after in vitro RNA removal, we identified here a large set of nuclear ERβ-interactors, including a sizeable fraction associating with the receptor via RNA bridging. Combining label-free quantitation and GO analysis revealed several cellular functions involving the nuclear interactome mapped here. The data presented will be useful for studies aiming at elucidating the role of ERβ, nuclear RNAs and the other proteins identified here in BC and other cell types.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11386/4703018
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 14
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 16
social impact