Physical adsorption preserving activity and support reusability was used to directly bond lipase from Thermomyces lanuginosus on citric acid (CA) modified Fe3O4/Au magnetic nanoparticles. A new faster approach has been used for CA ligand exchange, which ensures an high payload of stable enzyme. The immobilized lipase was tested for the biodiesel production from spent coffee ground in a solvent free system. It retains, after 60 days, more than 90% of its initial activity. Biodiesel yield of 51.7%, after 3 h of synthesis, which increases up to ∼100% after 24 h indicating an enzymatic fast kinetic, was measured. No significant decrease, during the first three cycles of use, of the lipase activity occurs. The biodiesel presents an ester content of 98.4 ± 0.23 in agreement with the EN14214. The iodine value of 76.67 (g iodine/100 g) is in agreement with the European standard.

Active biocatalyst for biodiesel production from spent coffee ground

Sarno, Maria
;
Iuliano, Mariagrazia
2018-01-01

Abstract

Physical adsorption preserving activity and support reusability was used to directly bond lipase from Thermomyces lanuginosus on citric acid (CA) modified Fe3O4/Au magnetic nanoparticles. A new faster approach has been used for CA ligand exchange, which ensures an high payload of stable enzyme. The immobilized lipase was tested for the biodiesel production from spent coffee ground in a solvent free system. It retains, after 60 days, more than 90% of its initial activity. Biodiesel yield of 51.7%, after 3 h of synthesis, which increases up to ∼100% after 24 h indicating an enzymatic fast kinetic, was measured. No significant decrease, during the first three cycles of use, of the lipase activity occurs. The biodiesel presents an ester content of 98.4 ± 0.23 in agreement with the EN14214. The iodine value of 76.67 (g iodine/100 g) is in agreement with the European standard.
2018
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11386/4718169
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 49
  • ???jsp.display-item.citation.isi??? 32
social impact