In this pilot study, we have explored the expression of RNA-binding proteins (RBPs), including AUF-1, tristetraprolin (TTP), and Human antigen R (HuR), in skin biopsies of atopic dermatitis (AD), compared to samples from patients with psoriasis and healthy subjects. We then investigated in vitro, using HaCaT cell line the effect of AD- and psoriasis-related cytokine stimulation on AUF- 1, TTP, and HuR expression. AUF-1 mRNA expression was evaluated in lesional and non-lesional skin of AD and psoriasis patients (n=5, respectively) and control donors (n=5). Protein levels of AUF-1, HUR, TTP, and AUF-1-regulated genes were evaluated by Western blot, ELISA, and RT-PCR in HaCat cells stimulated with interleukin (IL)-13, IL-4, IL-17 or TNF-α for 24h. RBP levels were next evaluated in a public microarray database of skin biopsies of lesional (n=12) and non-lesional (n=12) AD patients and controls (n=10). AUF-1 mRNA expression in AD skin (both lesional and non-lesional) was not changed compared to control subjects. Conversely, AUF-1 mRNA significantly increased in lesional psoriatic skin compared to controls and lesional AD. AUF-1 mRNA levels were slightly decreased in the AD lesional skin biopsies versus control biopsies in the GSE32924 database. Western blotting in HaCaT cells showed that treatment with AD-related cytokines IL-4 and IL-13 significantly decreased the expression of AUF-1 protein, while stimulation with psoriasis- related TNF-α and IL-17 significantly increased AUF-1 protein respect to unstimulated cells. HuR and TTP expression were not affected by any of the cell treatments. AUF-1-regulated genes, IL-6 and IL-1β, were significantly upregulated in IL-4+IL-13 stimulated HaCaT. Our results showed a different pattern of expression of AUF-1 in AD compared with psoriasis, suggesting a possible role of AUF-1 in chronic, immune-driven skin inflammation.
AUF-1 and skin inflammation: atopic dermatitis and psoriasis
S Lembo;A Raimondo;A Balestrino;C Stellato
2024-01-01
Abstract
In this pilot study, we have explored the expression of RNA-binding proteins (RBPs), including AUF-1, tristetraprolin (TTP), and Human antigen R (HuR), in skin biopsies of atopic dermatitis (AD), compared to samples from patients with psoriasis and healthy subjects. We then investigated in vitro, using HaCaT cell line the effect of AD- and psoriasis-related cytokine stimulation on AUF- 1, TTP, and HuR expression. AUF-1 mRNA expression was evaluated in lesional and non-lesional skin of AD and psoriasis patients (n=5, respectively) and control donors (n=5). Protein levels of AUF-1, HUR, TTP, and AUF-1-regulated genes were evaluated by Western blot, ELISA, and RT-PCR in HaCat cells stimulated with interleukin (IL)-13, IL-4, IL-17 or TNF-α for 24h. RBP levels were next evaluated in a public microarray database of skin biopsies of lesional (n=12) and non-lesional (n=12) AD patients and controls (n=10). AUF-1 mRNA expression in AD skin (both lesional and non-lesional) was not changed compared to control subjects. Conversely, AUF-1 mRNA significantly increased in lesional psoriatic skin compared to controls and lesional AD. AUF-1 mRNA levels were slightly decreased in the AD lesional skin biopsies versus control biopsies in the GSE32924 database. Western blotting in HaCaT cells showed that treatment with AD-related cytokines IL-4 and IL-13 significantly decreased the expression of AUF-1 protein, while stimulation with psoriasis- related TNF-α and IL-17 significantly increased AUF-1 protein respect to unstimulated cells. HuR and TTP expression were not affected by any of the cell treatments. AUF-1-regulated genes, IL-6 and IL-1β, were significantly upregulated in IL-4+IL-13 stimulated HaCaT. Our results showed a different pattern of expression of AUF-1 in AD compared with psoriasis, suggesting a possible role of AUF-1 in chronic, immune-driven skin inflammation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
