Fuel ethanol production by fermentation of cheese whey solution (lactose) can be a sustainable route for its high volume production worldwide and environmental pollution problem caused due to its high BOD(40-50 g.L−1) and COD(60 - 80 g.L−1) demand. Fermentation of cheese whey using free and immobilized yeasts Saccharomyces cerevisiae(Lewandowska et al., 2007), Kluyvromyces fragilis(Gianetto et al.,1986), Candida psudeotropicalis(Ghaly and El-Taweel,1997), Kluyveromyces marxianus (Ozmihci and Kargi, 2007, Zafar et al., 2005) has been reported with technical problems like poor substrate (lactose) utilization(in case of S.cerevisiae), substrate (lactose) and product (ethanol) inhibition affecting final ethanol concentration (batch) and ethanol productivity (continuous). Biofilm technology has been extensively applied in wastewater treatment, but its potential application in bioethanol production has not been explored. In general, advantages of biofilms include selective substrate and product diffusion due to layered microbial structure, prevention of cell wash out due to EPS (extra polymeric substance) formation, operational stability due to high resistance to external environment. This latter advantage is in particular sought in the bioethanol fermentation process. Kluyveromyces marxianus yeast strains has shown excellent ethanol tolerance (free and immobilized) and biofilm forming ability without any selective preference of support in the cited literature. The present research work involves studying the feasibility of anaerobic fermentation of cheese whey using Kluyveromyces marxianus DSMZ 5422 biofilm on particle support in batch and continuous mode. The first phase of the research involves characterization of Kluyveromyces marxianus DSMZ 5422 by fermentation of cheese whey powder solution at different concentration, pH and inoculum concentration. Natural supports such as olive pits and artificial supports such as polypropylene chips are tested for the holding capacity of Kluyveromyces marxianus DSMZ 5422 biofilm. Direct qualitative and indirect quantitative evaluation of the biofilm formation on the different support is carried out.

Bioethanol from cheese whey fermentation using Kluyveromyces marxianus biofilm

Joshi, Y
;
Poletto, M;Senatore, B
2010-01-01

Abstract

Fuel ethanol production by fermentation of cheese whey solution (lactose) can be a sustainable route for its high volume production worldwide and environmental pollution problem caused due to its high BOD(40-50 g.L−1) and COD(60 - 80 g.L−1) demand. Fermentation of cheese whey using free and immobilized yeasts Saccharomyces cerevisiae(Lewandowska et al., 2007), Kluyvromyces fragilis(Gianetto et al.,1986), Candida psudeotropicalis(Ghaly and El-Taweel,1997), Kluyveromyces marxianus (Ozmihci and Kargi, 2007, Zafar et al., 2005) has been reported with technical problems like poor substrate (lactose) utilization(in case of S.cerevisiae), substrate (lactose) and product (ethanol) inhibition affecting final ethanol concentration (batch) and ethanol productivity (continuous). Biofilm technology has been extensively applied in wastewater treatment, but its potential application in bioethanol production has not been explored. In general, advantages of biofilms include selective substrate and product diffusion due to layered microbial structure, prevention of cell wash out due to EPS (extra polymeric substance) formation, operational stability due to high resistance to external environment. This latter advantage is in particular sought in the bioethanol fermentation process. Kluyveromyces marxianus yeast strains has shown excellent ethanol tolerance (free and immobilized) and biofilm forming ability without any selective preference of support in the cited literature. The present research work involves studying the feasibility of anaerobic fermentation of cheese whey using Kluyveromyces marxianus DSMZ 5422 biofilm on particle support in batch and continuous mode. The first phase of the research involves characterization of Kluyveromyces marxianus DSMZ 5422 by fermentation of cheese whey powder solution at different concentration, pH and inoculum concentration. Natural supports such as olive pits and artificial supports such as polypropylene chips are tested for the holding capacity of Kluyveromyces marxianus DSMZ 5422 biofilm. Direct qualitative and indirect quantitative evaluation of the biofilm formation on the different support is carried out.
2010
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11386/4895036
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