The most common limitations of drug carriers, such as liposomes, are low Encapsulation Efficiency (EE), poor biocompatibility and drug degradation. Several techniques have been developed to overcome these problems; among these, Supercritical assisted Liposome formation (SuperLip) is a CO2 mediated technique for the production of liposomes at nanometric level. SuperLip has been employed to produce amoxicillin loaded liposomes. Vesicles have been loaded with 1, 5, 10 and 20 % (w/w) amoxicillin to lipid ratio; then, 1 % (w/w) cholesterol/lipid was added to liposomes lamellae. Liposomes showed mean diameters of about 200 nm and EE up to 84 %. Anti-microbial effect of Amoxicillin-loaded liposomes on Escherichia coli was studied. The growth of E.coli was monitored inoculating bacteria in Luria-Bertani medium with different liposomes concentrations (25, 50, 100 ppm) and different antibiotic/lipid ratio (5, 10, 20 %). An increase in the amoxicillin/lipid ratio enhanced growth inhibition of E.coli, especially at 100 ppm.

A supercritical assisted process for the production of amoxicillin loaded liposomes for anti-microbial applications

Trucillo P.;Reverchon E.;Perego P.
2020-01-01

Abstract

The most common limitations of drug carriers, such as liposomes, are low Encapsulation Efficiency (EE), poor biocompatibility and drug degradation. Several techniques have been developed to overcome these problems; among these, Supercritical assisted Liposome formation (SuperLip) is a CO2 mediated technique for the production of liposomes at nanometric level. SuperLip has been employed to produce amoxicillin loaded liposomes. Vesicles have been loaded with 1, 5, 10 and 20 % (w/w) amoxicillin to lipid ratio; then, 1 % (w/w) cholesterol/lipid was added to liposomes lamellae. Liposomes showed mean diameters of about 200 nm and EE up to 84 %. Anti-microbial effect of Amoxicillin-loaded liposomes on Escherichia coli was studied. The growth of E.coli was monitored inoculating bacteria in Luria-Bertani medium with different liposomes concentrations (25, 50, 100 ppm) and different antibiotic/lipid ratio (5, 10, 20 %). An increase in the amoxicillin/lipid ratio enhanced growth inhibition of E.coli, especially at 100 ppm.
2020
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11386/4754987
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 21
  • ???jsp.display-item.citation.isi??? 17
social impact